EFFECT OF ORAL ADMINISTRATION OF AQUEOUS STEM BARK EXTRACT OF KHAYA SENEGALENSIS ON HEPATOTOXICITY AND HYPERLIPIDEMIA IN RATS
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Date
2015-02-02
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Bayero University Kano
Abstract
The aim of this study is to investigate scientifically the basis for the use of aqueous stem
bark extract of K. senegalensis (ASBEKS) for the prevention of liver damage due to CCl4
hepatotoxicity and for the treatment of hyperlipidemia in hypercholesterolemic rats. A
total of seventy two rats were used in the study of which thirty six were used for testing
the hepatoprotective ability and were grouped into six groups of six rats each. Group one
served as normal control. Group two served as CCL4 induced control group, Group three
was administered with only ASBEKS at a dose of 2.10g/kg body weight per day for two
weeks. Group four, group five and group six were administered with the extract at a dose
of 1.05g/kg, 2.10gkg and 3.15g/kg respectively for two weeks. At the end of first week,
three rats from each group were selected, rats in groups II, IV, V and VI were induced
with liver damage using 120mg/kg of CCl4. The rats were sacrificed after 48hours of
CCl4 administration to assess liver function. At the end of the second week, same was
done to the remaining three rats from each group. Thirty six rats grouped into six groups
were used for testing the anti-hyperlipidemic effect of ASBEKS. Group II, IV, V and VI
were fed with high cholesterol rich diet to induce hypercholesterolemia. Group one
served as normal control. Group two served as hyperlipidemic control group, Group three
was administered only with ASBEKS at a dose of 2.10g/kg body weight per day. Group
four, group five and group six were hyperlipidemic and were administered with the
extract at a dose of 1.05g/kg, 2.10gkg and 3.15g/kg respectively. At the end of one week,
three rats from each group were sacrificed, at the end of the second week, the remaining
three rats from each group were also sacrificed and serum was collected for analysis of
serum lipid profile. A significant decrease (p<0.05) was observed in serum ALT, AST
and ALP of GROUP IV rats treated for one week when compared with the CCl4 induced
control, contrary to groups V and VI in the first week and groups III-VI in the second
week. There was significant (p<0.05) decrease in serum Total Cholesterol, LDLCholesterol and Triglyceride in hypercholesterolemic rats, with a concomitant increase in
HDL-cholesterol at dose of 1.05 g/kg, 2.10gkg and 3.15g/kg respectively in a dose
dependent manner even after the first week. The result clearly demonstrated
hepatoprotective and anti hyperlipidemic activity of Khaya senegalensis supporting the
traditional claim.