ANTI-CANCER STUDIES OF VITEX DONIANA AND FERETIA APODANTHERA EXTRACTS ON MCF-7 CELL LINE AND RATS INDUCED WITH BREAST CARCINOGENESIS
| dc.contributor.author | GILEAD EBIEGBERI FORCADOS | en |
| dc.creator | GILEAD EBIEGBERI FORCADOS | en |
| dc.date.accessioned | 2023-09-22T21:09:24Z | |
| dc.date.available | 2023-09-22T21:09:24Z | |
| dc.date.issued | 2019-12-26 | en |
| dc.description.abstract | Vitex doniana and Feretia apodanthera are two plants used traditionally for the treatment of inflammatory related disorders and cancer. This work investigated thein vitro antioxidant and in vitro cytotoxic effects of the extracts and fractions of V. doniana leaves and F. apodanthera root bark using diphenyl-β-picrylhydrazyl (DPPH), 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), hematoxylin and eosin staining, xCELLigence and fluorescence activated cell sorting assays. Subsequently,V. doniana whole extract was used for an in vivo study in female Wistar rats aged 52 ± 2 days administered a single dose of 80 mg/kg DMBA (dimethyl benz[a]anthracene) by oral gavage and monitored for 150 days. Rats with relatively high levels of serum estrogen receptor-α were randomly assigned to treatment groups administered 0, 50, 100, 200 mg/kg V. doniana and 20 mg/kg Tamoxifen for 14 days. The in vivo experiment was then terminated and serum estrogen receptor-α (ER-α), interleukin-1β (IL-1β) and tumour necrosis factor -α (TNF –α) levels were determined using ELISA kits, some oxidative stress markers in mammary tissue homogenates were determined and expression of cyclooxygenase-2 (COX-2), caspase 3 and p53 in mammary tissue was done using immunohistochemistry. LCMS analysiswas done on the V. doniana extract to determine possible compounds present. The highest free radical scavenging potency from DPPH assay was observed in V. doniana Fraction 2 which had an IC50 of 98.12 ± 4.94 μg/ml. MTT results showed an IC50 of 26.11 ± 3.4 μg/mL and 66.5 ± 5.2 μg/mL values for V. doniana and F. apodanthera respectively, after treatment for 48 hours. MCF-7 cells treated with V. doniana extract showed cell shrinkage, nuclear condensation, increased nuclear- cytoplasmic ratio and arrest at the G0/G1 phase of the cell cycle. In vivo, significantly. | en |
| dc.identifier.uri | https://teras.ng/api/asset/document/eda4820b-a359-4329-9832-ffe016852c09 | en |
| dc.identifier.uri | https://teras.ng/catalog-item/9daffe63-1ae1-482f-8c1d-ff2e8fd92f40 | en |
| dc.identifier.uri | http://dspace.teras-network.net:4000/handle/123456789/44379 | |
| dc.publisher | Ahmadu Bello University Zaria | en |
| dc.title | ANTI-CANCER STUDIES OF VITEX DONIANA AND FERETIA APODANTHERA EXTRACTS ON MCF-7 CELL LINE AND RATS INDUCED WITH BREAST CARCINOGENESIS | en |
| dc.type | Research Theses | en |
| thesis.degree.level | Masters | en |
| thesis.matric.number | P14SCBC9007 | en |