ENUMERATION OF MICROORGANISMS. IN DOMESTIC LOCAL WATER TANKER IN IJEBU - IGBO
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Date
2016-09-08
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Publisher
Abraham Adesanya Polytechnic Ijebu Igbo
Abstract
Many water utilities are required to monitor source water for the presence of total coliforms, fecal coliforms, or both. The Colilert system, an application of the
defined substrate technology, simultaneously detects the presence of both total
coliforms and Escherichia coli directly from a water sample. After incubation, the
formula becomes yellow if total colifonns are present and fluorescent at 366 nm if
E. coli is in the same sample. No confinnatory tests are required. The Colilert system
was previously assessed with distribution water in a national evaluation in both
most-probable-number and presence-absence formats and found to produce data
equivalent to those obtained by using Standard Methods for the Examination of
Water and Wastewater (APHA, 1985). The Colilert system was now compared with
Standard Methods Multiple-Tube Fermentation (MTF) for the enumeration of total
colifon11S and E. coli from surface water. All MTF tubes were confirmed according
to Standard Methods, and subcultures were made to identify isolates to the species
level. Colilert tubes were sub-cultured to determine if color changes were specific
to the target microbes. The Colilert system was found equally sensitive to MTF
testing by regression, t-test, chi-square, and likelihood fraction analyses. Specificity
of the Colilert system was shown by the isolation of a species of total coliform or
E. coli after the appropriate colour change. The Colilcrt test can be used for source
water samples when enumeration is required, and the benefits previously described
for distribution water testing-sensitivity, specificity, less labor, lower cost, faster
results, and no non-coliform heterotroph interference-are applicable to this type of
water analysis. This study demonstrates that there is prevalence of microorganism
can be found in water tanker and should be treated more regularly or often to prevent
consumers or user from contacting these coliform disease which is harmful to human lung