EVALUATION OF ANTI-INFLAMMATORY EFFECTS OF AQUEOUS LEAF EXTRACT OF SYZYGIUM GUINEENSE ON CARRAGEENAN-INDUCED PAW OEDEMA IN ADULT MALE WISTAR RATS
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Date
2021-07-27
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Ahmadu Bello University Zaria
Abstract
Inflammation is implicated in many disease conditions, such as cardiovascular diseases, diabetes
mellitus and cancer. Syzygiumguineensehas been reported to contain active phytochemicals that
may inhibit the inflammatory responses, decrease the levels of inflammatory mediators and
attenuate the abnormal formation of reactive oxygen metabolites. The aim of this study is to
determine the Anti-Inflammatory Effects of Aqueous Leaf Extract of Syzygiumguineenseon
Carrageenan-induced Paw Oedema inAdult Male Wistar Rats.At the beginning of the study acute
toxicity studies was carried out, which showed that the aqueous leaf extract of Syzygium guineense
is safe to use since no mortality was recorded and no signs of toxicity was developed by the animals
in each phases of acute toxcity studies. The preliminary phytochemical analysis was also conducted
and variuos active agents were present in aqueous leaf extract of Syzygium.Thirty albino wistar rats
were used for the study, the animals were divided into six (6) groups of five (5) rats each. The
animals were grouped as follows: Group I received normal saline 1mg/kg only. Group II receive
carrageenan injection only. Group III wasadministered with Diclofenac 10mg/kg orally. Group IV
receieved 250mg/kg, Group V received 500mg/kg, and Group VI received 1000mg/kg
ofSyzygiumguineense per orally. One hour after the administrations, each rat except group I, was
injected with 0.1ml of carragenan (1%w/v) into the subplanter region of the right hind paw. All the
three doses of aqueous leaf extract of Syzygiumguineense, and Diclofenac 10mg/kgsignificantly
(P≤0.05) inhibited the Carrageenan induced paw oedema after 3 hours. The anti-oxidant enzymes,
SOD,CAT and GSH levels were not significantly (P≤0.05) increased
bySyzygiumguineensecompared to carrageenan group, but the 250mg/kg dose increase the SOD and
CAT levels compared to normal saline group. The 250mg/kg ofSyzygium guineense caused
statistically significant (P≤0.05) increase in Neutrophil countscompared Carrageenan and normal