STUDIES ON RESISTANCE GENES ASSOCIATED WITH Staphylococcus aureus IN WOUND INFECTIONS AMONG PATIENTS ATTENDING MURTALA MUHAMMAD  SPECIALIST HOSPITAL (MMSH), KANO

FAIZA MUSA

STUDIES ON RESISTANCE GENES ASSOCIATED WITH Staphylococcus aureus IN WOUND INFECTIONS AMONG PATIENTS ATTENDING MURTALA MUHAMMAD SPECIALIST HOSPITAL (MMSH), KANO

Date

2017-03-01

Authors

FAIZA MUSA

Publisher

Bayero University Kano

Abstract

This study was carried out to determine the antibiotic susceptibility pattern of Staphylococcus aureus isolated from wounds and to study some of the resistance genes. The study was conducted on patients with wound infection attending Murtala Muhammad Hospital, Kano. A sterile swab stick was used to collect the sample before wound dressing and it was cultured on mannitol salt agar (MSA). Biochemical test was carried out to identify the S. aureus followed by MRSA screening using cefoxitin (30mcg) disc. Antibiotic susceptibility pattern of the isolates was determine using agar diffusion method. PCR was carried out to detect the resistant genes. Out of the 180 wound swabs collected S. aureus was isolated from 51 (28%), and all were sensitive to ciprofloxacin. Methicillin resistance Staphylococcus aureus (MRSA) was found to be 19.61%. There was no significant difference between S. aureus isolated among age groups (P>0.05). There was significant difference in S. aureus isolated between different wound type (P< 0.05). Five (9.8%) of the isolates were screened for the presence of MecA and FemB gene. MecA gene was found in 20% of the screened samples and FemB was also found in 20% of the screened samples. Occurance of FemB and MecA genes among the isolates should be considered as a menace that could cause havoc to effective chemotherapy of wound infections in the population studied.