Evaluation of Azadirachta indicaextracts invitro on the growth and development of Phytophthtora megllkarya causal organism of blackpod disease of cocoa (Tlteobroma cacao L.)
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Date
2016-10-13
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Abraham Adesanya Polytechnic Ijebu Igbo
Abstract
The efficacy of Azadirachtan indica Neem leaf extracts were evaluated on the growth and development of P. mega aryam vitro using acetone, ethanol and
methanol as extraction solvent.Fourier Tranform Infra-Red (FTIR)
Spectrometer was used to determine the quantitative constituents of the plant extracts. Five levels of crude extracts concentrations were used viz; 20, 40, 60, 80 and 100% respectively to determine the best concentration at which the
extract will be most effective against the pathogen.The fungus Phytophthora
megakarya was isolated from infected cocoa pod. Food poisoning technique
was used to determine the toxicity of the neem extracts against the grov.·th of
the pathogen.5 ml of A. indica extracts were mixed with 20 ml of Potato
Dextrose Agar (PDA) to get 20, 40, 60, and 80 % concentration, while the
undiluted extract was recorded as I 00 %. The plates were inoculated with a
5mm assay disc cut from 7 day old culture fungus; data were taken starting
from ¡ day after inoculation and the experiment was terminated at 7'h day after inoculation.